e·E&E BIO CLUB
PRODUCT CATALOGUE

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✳︎PRODUCTS
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01 · PRODUCTS

mRNA

6 PRODUCTS
A

Fluorescent reporters

3 products
R0201mRNA

mCherry mRNA

mCherry reporter mRNA.

Vero cells transfected with mCherry mRNA
Vero cells transfected with mCherry mRNA.
Quantity 1 mgListed price $999.00
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R0202mRNA

TurboGFP mRNA

TurboGFP reporter mRNA.

Vero cells transfected with turboGFP mRNA
Vero cells transfected with turboGFP mRNA.
Quantity 1 mgListed price $999.00
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C

Bioluminescent reporters

3 products
R0206mRNA

Gaussia luciferase mRNA

Gaussia luciferase reporter mRNA.

Quantity 1 mgListed price $999.00
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R0207mRNA

Cypridina luciferase mRNA

Cypridina luciferase reporter mRNA.

Quantity 1 mgListed price $999.00
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R0208mRNA

Firefly luciferase mRNA

Firefly luciferase reporter mRNA.

Quantity 1 mgListed price $999.00
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02 · PRODUCTS

Genomic DNA

2 PRODUCTS
A

Cell line reference DNA

2 products
03 · PRODUCTS

Plasmid DNA

26 PRODUCTS

These catalog plasmids are off-the-shelf templates for modification or as starting material for further large-scale preparation. For purification of your own plasmid samples, see our plasmid preparation service.

A

In vitro transcription (IVT)

9 products
P0401Plasmid DNA

mCherry mRNA IVT vector

DNA vector for in vitro transcription of mCherry mRNA, with a 208-bp polyA tail, HindIII restriction site, and T7 terminator.

More product details

For co-transcriptional cap analog incorporation, use CleanCap AG. For enzymatic capping by vaccinia capping enzymes or faustovirus capping enzyme, nucleotide A is not a suitable substrate under in vitro conditions. Contact us to request a different version.

Quantity 20 µgListed price $220.00
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P0402Plasmid DNA

TurboGFP mRNA IVT vector

DNA vector for in vitro transcription of TurboGFP mRNA, with a 208-bp polyA tail, HindIII restriction site, and T7 terminator.

More product details

For co-transcriptional cap analog incorporation, use CleanCap AG. For enzymatic capping by vaccinia capping enzymes or faustovirus capping enzyme, nucleotide A is not a suitable substrate under in vitro conditions. Contact us to request a different version.

Quantity 20 µgListed price $220.00
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P0403Plasmid DNA

Sleeping Beauty Transpose mRNA IVT vector

DNA vector for in vitro transcription of a bicistronic mRNA encoding EGFP and Sleeping Beauty Transposase.

More product details

Monitor successful mRNA transfection and duration by the EGFP fluorescent signal; low SB Transposase expression mediated by IRES reduces removal events of the already transposed transgene from the genome. For co-transcriptional cap analog incorporation, use CleanCap AG. RNA produced with this vector is not suitable for in vitro enzymatic capping.

Quantity 20 µgListed price $220.00
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P0404Plasmid DNA

Cypridina luciferase mRNA IVT vector

DNA vector for in vitro transcription of Cypridina luciferase mRNA, with a 208-bp polyA tail, HindIII restriction site, and T7 terminator.

More product details

For co-transcriptional cap analog incorporation, use CleanCap AG. For enzymatic capping by vaccinia capping enzymes or faustovirus capping enzyme, nucleotide A is not a suitable substrate under in vitro conditions. Contact us to request a different version.

Quantity 20 µgListed price $220.00
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P0405Plasmid DNA

Flippase mRNA IVT vector

DNA vector for in vitro transcription of Flippase mRNA, with a 208-bp polyA tail, HindIII restriction site, and T7 terminator.

More product details

For co-transcriptional cap analog incorporation, use CleanCap AG. For enzymatic capping by vaccinia capping enzymes or faustovirus capping enzyme, nucleotide A is not a suitable substrate under in vitro conditions. Contact us to request a different version.

Quantity 20 µgListed price $220.00
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P0406Plasmid DNA

Gaussia luciferase mRNA IVT vector

DNA vector for in vitro transcription of Gaussia luciferase mRNA, with a 208-bp polyA tail, HindIII restriction site, and T7 terminator.

More product details

For co-transcriptional cap analog incorporation, use CleanCap AG. For enzymatic capping by vaccinia capping enzymes or faustovirus capping enzyme, nucleotide A is not a suitable substrate under in vitro conditions. Contact us to request a different version.

Quantity 20 µgListed price $220.00
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P0407Plasmid DNA

Firefly luciferase mRNA IVT vector

DNA vector for in vitro transcription of Firefly luciferase mRNA, with a 208-bp polyA tail, HindIII restriction site, and T7 terminator.

More product details

For co-transcriptional cap analog incorporation, use CleanCap AG. For enzymatic capping by vaccinia capping enzymes or faustovirus capping enzyme, nucleotide A is not a suitable substrate under in vitro conditions. Contact us to request a different version.

Quantity 20 µgListed price $220.00
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P0408Plasmid DNA

mCherry mRNA IVT vector

DNA vector for in vitro transcription of mCherry mRNA, with a 195-bp polyA tail, HindIII restriction site, and T7 terminator.

More product details

For co-transcriptional cap analog incorporation, use ARCA. RNA produced with this vector is suitable for enzymatic capping.

Quantity 20 µgListed price $220.00
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P0409Plasmid DNA

TurboGFP and puromycin resistance IVT vector

DNA vector for in vitro transcription of TurboGFP and puromycin resistance gene, with a 208-bp polyA tail, MluI restriction site, and T7 terminator.

More product details

For co-transcriptional cap analog incorporation, use ARCA. RNA produced with this vector is suitable for enzymatic capping.

Quantity 20 µgListed price $220.00
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B

Lentiviral vector packaging

4 products
P0410Plasmid DNA

Third-generation lentiviral mCherry vector

DNA vector for expressing genomic RNA of a third-generation lentiviral vector encoding an mCherry reporter gene.

Quantity 20 µgListed price $220.00
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P0411Plasmid DNA

Lentiviral packaging helper vector · VSV-G

Lentiviral vector packaging helper vector expressing VSV-G.

Quantity 20 µgListed price $220.00
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P0412Plasmid DNA

Lentiviral packaging helper vector · rev

Lentiviral vector packaging helper vector expressing rev.

Quantity 20 µgListed price $220.00
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P0413Plasmid DNA

Lentiviral packaging helper vector · Gag/Pol

Lentiviral vector packaging helper vector expressing Gag/Pol.

Quantity 20 µgListed price $220.00
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C

AAV2 packaging

3 products
P0414Plasmid DNA

AAV2 construct · CMV promoter

DNA construct for AAV2 with a CMV promoter and polyA signal flanked by AAV2 ITRs.

Quantity 20 µgListed price $220.00
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P0415Plasmid DNA

AAV2 Rep and Cap helper vector

Helper vector for AAV2 packaging encoding AAV2 Rep and AAV2 Cap.

Quantity 20 µgListed price $220.00
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P0416Plasmid DNA

Adenovirus helper plasmid · E2A and E4

Helper plasmid for packaging AAV vectors, encoding Ad5 E2A and Ad5 E4.

Quantity 20 µgListed price $220.00
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D

VSV recovery

5 products
P0417Plasmid DNA

VSV mCherry antigenomic RNA plasmid

DNA plasmid encoding the antigenomic RNA of VSV expressing an mCherry reporter gene.

Quantity 20 µgListed price $220.00
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P0419Plasmid DNA

VSV L gene helper plasmid

The helper plasmid encodes the VSV L gene needed to rescue recombinant VSV.

Quantity 20 µgListed price $220.00
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P0420Plasmid DNA

VSV N, P, and L gene helper plasmid

The DNA plasmid encodes the VSV N, P, and L genes required to rescue recombinant VSV. These three genes are organized into a tricistronic mRNA. Translation of the N gene is cap-dependent, whereas translation of the P and L genes is initiated by distinct Internal Ribosome Entry Sites (IRESs).

Quantity 20 µgListed price $220.00
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P0421Plasmid DNA

VSV N gene expression plasmid · T7

The helper plasmid encodes the VSV N gene needed to rescue recombinant VSV.

Quantity 20 µgListed price $220.00
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P0422Plasmid DNA

VSV P and L gene expression plasmid · T7

The helper plasmid encodes the VSV P and L genes required to rescue recombinant VSV. P and L are encoded by a bicistronic mRNA.

Quantity 20 µgListed price $220.00
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E

Cell line engineering

4 products
P0423Plasmid DNA

Sleeping Beauty gene integration construct

DNA construct for genome integration of the gene of interest (GOI) via flippase. The vector contains the reporter gene turboGFP and the puromycin resistance gene as the selection marker, both driven by the RSV promoter and separated by the P2A self-cleavage peptide. The CAGG promoter controls GOI expression. The GOI encoded by this vector is the mCherry reporter gene. Genome integration requires a pre-established cell line with an engineered FRT site, such as C0601 (derived from Vero cells). We accept requests for custom engineering of other cell lines.

Quantity 20 µgListed price $220.00
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P0424Plasmid DNA

Flippase-mediated gene integration construct

DNA construct for random genome integration of the gene of interest (GOI) using the Sleeping Beauty transposon. The vector contains the reporter gene turboGFP and the zeocin resistance gene as the selection marker, both driven by the RSV promoter and separated by an Internal Ribosome Entry Site (IRES).

Quantity 20 µgListed price $220.00
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P0425Plasmid DNA

AAVS1 CRISPR donor vector

AAVS1 donor vector for transgene integration by CRISPR, containing AAVS1 homologous arms.

More product details

The gene of interest can be cloned downstream of the CAGG promoter.

Quantity 20 µgListed price $220.00
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P0426Plasmid DNA

FRT-site cell engineering vector

DNA vector for engineering cell lines with an FRT site. The vector contains two Inverted Terminal Repeats of the Sleeping Beauty transposon, which mediates genome integration. The selection marker is TurboGFP and Zeocin, flanked by two FRT sites. The selection marker can be removed by introducing flippase, leaving one FRT site available for flippase-mediated transgene integration.

Quantity 20 µgListed price $220.00
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O

Other

1 product
P0431Plasmid DNA

Self-amplifying RNA TurboGFP vector

DNA vector for self-amplifying RNA expressing turboGFP.

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04 · PRODUCTS

Viral Vector

1 PRODUCT
A

VSV reporter vector

1 product
V0501Viral Vector

VSV expressing mRNA reporter gene

VSV expressing an mRNA reporter gene.

Quantity 10⁹ PFUListed price $450.00
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05 · PRODUCTS

Cell Line

2 PRODUCTS
A

Engineered cell line

2 products
C0601Cell Line

Engineered Vero cells with an FRT site

Engineered Vero cells with a specific Flp recognition target (FRT) site integrated into the genome.

Quantity 10⁶ cellsListed price $998.00
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C0602Cell Line

Engineered Vero cells expressing PD-L1 and TurboGFP

An engineered Vero cell line that constitutively expresses PD-L1, TurboGFP, and a puromycin resistance gene. The reporter and puromycin resistance genes are flanked by Flippase FRT sites and can be excised by introducing Flippase.

Quantity 10⁶ cellsListed price $998.00
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